Role of CD46 in Measles Virus Infection in CD46 Transgenic Mice
Identifieur interne : 000289 ( France/Analysis ); précédent : 000288; suivant : 000290Role of CD46 in Measles Virus Infection in CD46 Transgenic Mice
Auteurs : Merete Blixenkrone-M Ller [France] ; Arlette Bernard [France] ; Anne Bencsik [France] ; Nathalie Sixt [France] ; Lisa E. Diamond ; John S. Logan ; T. Fabian Wild [France]Source :
- Virology [ 0042-6822 ] ; 1998.
English descriptors
- Teeft :
- Academic press, Animal model, Becton dickinson, Brain tissue, Canine distemper virus, Canine distempter virus, Cell culture supernatants, Cell cultures, Cell lines, Cell lysates, Cerebellum, Cerebral edema, Clinical symptoms, Cofactor, Cortex hippocampus cerebellum, Cryo sections, Culture supernatants, Cultured cells, Different structures, Edmonston, Edmonston inoculation, Edmonston strain, Edmonston strains, Experimental period, Facs buffer, Fetal calf serum, First week postinoculation, Frontal cortex, Glioma cell line, Halle, Halle strain, Hela cells, Hippocampus, Human membrane cofactor protein, Human origin, Infection, Infection studies, Infectious virus, Inoculated, Inoculated intraperitoneally, Inoculation, Intracerebral, Intracerebral inoculation, Intranasal infection, Lung cell cultures, Lung tissue, Lyon cedex, Mabs, Measles, Measles virus, Measles virus binding, Measles virus infection, Measles virus replication, Membrane cofactor protein, Mice inoculated, Microscope slides, Mouse, Mouse brains, Mouse cell cultures, Murine, Negative controls, Nontransgenic, Nontransgenic cells, Nontransgenic mice, Normal mouse, Normal rabbit serum, Pbmc, Peripheral blood, Permissive host cells, Positive controls, Postinoculation, Present study, Primary antibody, Primer pair, Receptor, Receptor interactions, Replication, Replicative cycles, Same pattern, Secondary antibody, Serum samples, Southern blot, Sspe, Supernatant, Syncytia formation, Syncytium, Tissue sections, Transgenic, Transgenic cells, Transgenic mice, Transgenic mice measles virus, Transgenic mouse, Transgenic mouse cells, Transgenic rats, Vaccine, Vero, Vero cell cultures, Vero cells, Viral, Viral antigen, Viral replication, Viral transcripts, Virol, Virus, Virus infection, Virus isolation, Yagamata sspe strain, Yamagata, Yamagata strain.
Abstract
Abstract: The susceptibility of CD46 (human membrane cofactor protein) transgenic mice to measles virus (MV) infection was investigated. Cell cultures (lung and kidney) established from transgenic and control mice showed that although both could be infected only those from the CD46+ mice gave fusion. A complete round of replication with the release of infectious virus was detected exclusively in the transgenic cell cultures whose permissiveness to MV was markedly less than that of Vero cells. The ability of MV to replicatein vivoin mice was studied using both vaccine and laboratory-adapted wild-type strains of virus. After intraperitoneal and intranasal inoculations of transgenic mice, virus replication could not be detected. In contrast intracerebral inoculation induced infection in both transgenic and nontransgenic mice. Our results fromin vitroinfection studies support the hypothesis that CD46 is a major host cell factor involved in the MV-induced fusion process and MV entry. The studies further indicate that MV tropism is not governed solely by the expression of the CD46 gene and that the high efficiency of the replicative cycles characteristic of fully permissive host cells requires additional factors, which are lacking in both transgenic and nontransgenic mice.
Url:
DOI: 10.1006/viro.1998.9301
Affiliations:
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Cell cultures (lung and kidney) established from transgenic and control mice showed that although both could be infected only those from the CD46+ mice gave fusion. A complete round of replication with the release of infectious virus was detected exclusively in the transgenic cell cultures whose permissiveness to MV was markedly less than that of Vero cells. The ability of MV to replicatein vivoin mice was studied using both vaccine and laboratory-adapted wild-type strains of virus. After intraperitoneal and intranasal inoculations of transgenic mice, virus replication could not be detected. In contrast intracerebral inoculation induced infection in both transgenic and nontransgenic mice. Our results fromin vitroinfection studies support the hypothesis that CD46 is a major host cell factor involved in the MV-induced fusion process and MV entry. The studies further indicate that MV tropism is not governed solely by the expression of the CD46 gene and that the high efficiency of the replicative cycles characteristic of fully permissive host cells requires additional factors, which are lacking in both transgenic and nontransgenic mice.</div></front></TEI><affiliations><list><country><li>France</li></country><region><li>Auvergne-Rhône-Alpes</li><li>Rhône-Alpes</li></region><settlement><li>Lyon</li></settlement></list><tree><noCountry><name sortKey="Diamond, Lisa E" sort="Diamond, Lisa E" uniqKey="Diamond L" first="Lisa E." last="Diamond">Lisa E. Diamond</name><name sortKey="Logan, John S" sort="Logan, John S" uniqKey="Logan J" first="John S." last="Logan">John S. Logan</name></noCountry><country name="France"><region name="Auvergne-Rhône-Alpes"><name sortKey="Blixenkrone M Ller, Merete" sort="Blixenkrone M Ller, Merete" uniqKey="Blixenkrone M Ller M" first="Merete" last="Blixenkrone-M Ller">Merete Blixenkrone-M Ller</name></region><name sortKey="Bencsik, Anne" sort="Bencsik, Anne" uniqKey="Bencsik A" first="Anne" last="Bencsik">Anne Bencsik</name><name sortKey="Bernard, Arlette" sort="Bernard, Arlette" uniqKey="Bernard A" first="Arlette" last="Bernard">Arlette Bernard</name><name sortKey="Sixt, Nathalie" sort="Sixt, Nathalie" uniqKey="Sixt N" first="Nathalie" last="Sixt">Nathalie Sixt</name><name sortKey="Wild, T Fabian" sort="Wild, T Fabian" uniqKey="Wild T" first="T. Fabian" last="Wild">T. Fabian Wild</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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